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Author contributions: M. Water channel aquaporin 4 AQP4 plays a key role in the regulation of water homeostasis in the brain. It is predominantly expressed in astrocytes at the blood—brain and blood—liquor interfaces. Although several AQP4 isoforms have been identified in the mammalian brain, two, AQP4a M1 and AQP4c M23 , have been confirmed to cluster into plasma membrane supramolecular structures, termed orthogonal arrays of particles OAPs and to enhance water transport through the plasma membrane.
However, the role of the newly described water-conductive mammalian isoform AQP4e is unknown. Here, the dynamics of AQP4e aggregation into OAPs and its role in the regulation of astrocyte water homeostasis have been studied. Using super-resolution structured illumination, atomic force, and confocal microscopies, the results revealed that, in female rat astrocytes, AQP4e isoform colocalizes with OAPs, affecting its structural dynamics. Moreover, the kinetics of the cell swelling and of the regulatory volume decrease was faster in astrocytes overexpressing AQP4e compared with untransfected controls.
Furthermore, the increase in maximal cell volume elicited by hypoosmotic stimulation was significantly smaller in AQP4e-overexpressing astrocytes. For the first time, this study demonstrates an active role of AQP4e in the regulation of OAP structural dynamics and in water homeostasis.
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The data records will follow and are the same as they have been all along, with the exception that tas is no longer encoded. Additional meta-data may present. The PMS-2D raw data comes in 4 kilo-byte records of 32 bit slices from the probe slices. The interface card then wraps a record header around that with time stamp and other pertinent information.
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OAP Application Workshop will guide you through the application process and required items to apply for Other UC & Non-UC programs. 8/27 11am.
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About The Journal. All submissions can be done at our Online Manuscript Submission System. Authors are requested to submit their articles directly to Online Manuscript Submission. Acupuncture therapy, Anthroposophic medicine, Anthroposophy, Auriculotherapy, Auto immune diseases, Ayurvedic medicine, Bio resonance therapy, Chromotherapy, Complementary medicine, Diffuse noxious inhibitory control, Holistic health, Holistic medicine, Homeopathy, Horticultural therapy, Mesotherapy etc.
Remaining Scope can be viewed at Aim and Scope. Manuscript template: Title, List of authors, their affiliations and email addresses, Abstract, Introduction, Main body, Results and discussion, Conclusions, Acknowledgments optional , References. Formatting guidelines can be found at Instructions for Authors. Hence, the operation of the journal is supported by the processing fees received from authors.
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